No. At least not in my life experience. There's not much reason why you can't use both to mean the same thing.
Is there a difference between the two? Sure, something you do in your free time like eating food may not be a hobby. "What do you do in your free time" could mean hobbies, but also life needed tasks chores like shopping, cleaning, hygiene, caring for children or husbands/wives, ect. While "What's your hobby" is explicit, and is almost always done in free time.
In observed at least American talking culture, what people mean when they ask, "What do you do in your free time" they very likely mean "What do you do for a hobby", because most people (I would think) would not treat chores as hobbies. Unless you really are that interested in cleaning.