The literal meaning of 'dust off' is to remove the dust from something.
We often use it as an idiom to indicate that you haven't done something for a long time. For example, you might say that you're going to dust off your running shoes to show that you're going to take up running again after a long period of being lazy.
You can also it use idiomatically for something which can't literally gather dust, for example, your language skills.