If someone is not mindful of your personal space and you want them to give you space, you could say "Please step back" or "Step back, please."
**depending on your tone, it can be interpreted as a peaceful request or rude.
-"Back off" is typically interpreted as rude and is used when in heated discussion with disregard to personal space.
-"Back up" can be interpreted as rude, but you could also say "Please back up" or "Back up, please" to make it a polite request.
**Tone is most important.**
I hope this helps you!