They have fairly similar meanings but they can come across in different ways in different situations. Firstly, you can use 'don't touch me' as a warning before someone touches you, as well as when someone is already touching you. You can only use 'get your hands off me' when someone is already touching you. Secondly, they can be different in tone. 'Don't touch me' could be said in many different ways. You could say it calmly to someone to set a boundary, or you could say it more urgently depending on the situation. 'Get your hands off me' is usually said in a more urgent situation when someone feels uncomfortable and can come across as a more aggressive thing to say. In basic terms, 'get your hands off me' is used in more serious situations, and 'don't touch me' can be used differently depending on the situation.

There is a difference: * "Don’t touch me": A general command to not make physical contact. * "Get your hands off of me": Implies the person is already touching you, and you're telling them to stop. So, the first prevents contact, while the second demands stopping contact.

They can mean practically the same thing in some situations. For example, if someone puts their hands on you in a way that makes you uncomfortable, then either of those instructions would be suitable and mean practically the same thing. But technically there is a difference in meaning. "Don't touch me" literally means "do not touch me" which means now and in future. It does not suggest you've already been touched, but you may have been. And it does not say how you may be touched, it just means don't touch me in any way whatsoever. "Get your hands off of me" is a bit different, as it implies someone has already started touching you, specifically with their hands, and it's specifically that form of contact that you demand they stop. So, this is a bit more of a detailed instruction, which may be a significant difference in some situations, but in other situations has negligible difference.